Antimicrobial activity of earthworm extracts (2025)

Antimicrobial activity of earthworm (Eudrilus eugeniae) paste

African Journal of Environmental Science and Technology, 2013

Earthworm plays a major role in the proper functioning of the soil ecosystem. It acts as scavenger and helps in recycling of dead and decayed plant material by feeding on them. Earthworm increases the soil fertility and is often referred to as a farmer's friend. Earthworms have been used in medicine for various remedies. The paste prepared from earthworm, Eudrilus eugeniae was tested for antibacterial, antifungal activities. For the antimicrobial screening, four species of bacterial isolate and two species of fungal isolates were selected. The bacterial cultures were used for antimicrobial testing maintained on nutrient agar slant and the fungal strains were maintained on Sabouraud dextrose agar slant at 4°C. Minimum inhibitory concentration (MIC) was determined using micro dilution broth method. Earthworm paste at a dose of 100 µl was able to inhibit the growth of bacteria of S. aureus at a maximum level as compared to other bacteria; the growth of fungal Candida albicans was much inhibited. The MIC results indicated that earthworm paste at a dose of 200 µl inhibited the bacterial growth. These studies may lead to the formulation of new antimicrobial drug. The antimicrobial activity of the paste was determined by an agar diffusion method using well and disc, the study clearly indicates that the paste contain a good antibacterial potential and the bioactive compounds to inhibit the growth of bacteria and fungi. Hence earthworm paste (EP) has a good potential to develop a new drug.

Evaluation of antimicrobial activity if earthworm t issue extract of Eudrilus eugeniae

2014

As earlier reported that the defense system of eart hworm is found in their coelomic fluid. Different p roteins and peptides (lysozyme and fetidins) have been isolated in different species of earthworm, responsible for their antibacterial activity. Antibacterial & antifungal activities of the whole tissue extract of earthworm , Eudrilus eugeniae was tested using well diffusion method. Ni ne species are selected for antimicrobial screening and two species are for antifungal screening .The bacterial cultures were maintained on nutrient agar slant an d the fungal strains were maintained on Sabovraud dextrose agar slant at 4°C as well as at -20°C by making glycerol stocks. Earthworm paste at a dose of 10mg/ml was able to in hibit the growth of bacteria. Among nine bacterial isolates only five species responded to earthworm extract. M aximum antibacterial activity was found with gram n Pseudomonas aeruginosa (29 ± 1mm), which was almost similar to the standard antibiotic Streptomycin. (0...

Evaluation of antimicrobial activity of earthworm Eudrilus eugeniae

2015

As earlier reported that the defense system of eart hworm is found in their coelomic fluid. Different p roteins and peptides (lysozyme and fetidins) have been isolated in ifferent species of earthworm, responsible for their antibacterial activity. Antibacterial & antifungal activities of the whole tissue extract of earthworm , Eudrilus eugeniae was tested using well diffusion method. Ni ne species were selected for antimicrobial screenin g a d two for antifungal screening. The bacterial cultures were m aintained on nutrient agar slant and the fungal str ains were maintained on Sabouraud dextrose agar slant at 4°C as well as at -20°C by making glycerol stocks. Eart hworm paste at a dose of 10mg/ml was able to inhibit the growth of bacteria. Among nine bacterial isolates o nly five species responded to earthworm extract. Maximum ant ib cterial activity was found with gram negative Pseudomonas aeruginosa (29 ± 1mm), which was almost similar to the standard antibiotic Streptomycin. ( 29...

Anti-inflammatory activity of earthworm extracts

Soil Biology and Biochemistry, 1992

Earthworm plays a major role in the proper functioning of the soil ecosystem. It acts as scavenger and helps in recycling of dead and decayed plant material by feeding on them. Earthworm increases the soil fertility and is often referred to as a farmer's friend. Earthworms have been used in medicine for various remedies. In the present investigation, various solvent extracts of an earthworm, Eudrilus eugeniae were prepared and antiinflammatory activity of these extracts were determined. The petroleum ether fraction possessed maximum anti-inflammatory activity in carrageenan induced albino rats in comparison to 95% ethanol and 0.2 M phosphate buffer (pH, 7.0) extracts. The paw volume was determined and was compared with that of aspirin, a standard anti-inflammatory drug. The results indicates that petroleum ether fraction of earthworm extract possessed similar anti-inflammatory activity as that of aspirin.

Comparative Studies on terrestrial and marine Earthworm extracts against Human pathogens

The present investigation was studied to screening the bioactive compounds agaisnt human pathogens from Terrestrial and Marine earthworms extracts from in and around terrestrial area of Sivaganga district and the marine earth worms collected from the coastal area of Thondi, Ramanathapuram. The antibacterial activity was determined by measuring the diameter of zone of inhibition recorded. Ethanolic extract of earthworm possessed maximum antibacterial activity in comparison to marine earthworm extract against antibiotic resistant pathogens of Staphylococcus aureus. The diameter of zone of inhibition observed was 19mm. In addition that, terrestrial and marine earth warm extract were screening urinary tract infections pathogens. Results show that, maximum activity was observed against Proteus morganii by marine earth worm extract. The zone of inhibition observed was 14 mm.Moreover, the both terrestrial and earth worm extract have shown antibacterial activity at the used concentrations. The present investigation showed that, maximum inhibition was observed in 1000 mg concentration of marine earthworm extract agaisnt Streptococcus pyogens at 15 nm. In the current work, we have collected terrestial and marine of intestinal fluid. From the study it is evident that the earthworm extracts possess antimicrobials properties. Keywords: Marine and terrestrial earthworm, Bioactive compounds, Human pathogens,

ANTIBACTERIAL ACTIVITY ETHYL ACETATE EXTRACTS OF EARTHWORMS (Lumbricus rubellus, Eisenia foetida, Nereis sp) TOWARD Staphylococcus aureus, Enterococcus faecalis, Salmonella thyposa INVITRO

El-Hayah

Earthworms had a mechanism of antibacterial. The research aimed to observe Lumbricus rubellus, Eisenia foetida, Nereis sp. antibacterial activity against Salmonella thyposa, Staphylococcus aureus, Enterococcus faecalis in vitro compared to ampicillin antibiotics. All the worms extracted using ethyl acetate extraction and tested their MIC. The compound of amino acids of the worms was analyzed by HPLC and nanodrop. Lumbricus rubellus was the best anti-bacteria activity followed by Eisenia foetida and Nereis sp., but these activities less than ampicillin antibiotic. Observations with SEM showed these worms extract caused cell leakage in all of these bacteria. Protein content with Nanodrop testing revealed the highest protein content was Lumbricus rubellus (21.75 ppm) followed by Eisenia foetida (21.32 ppm) and Nereis sp. (20.98 ppm), as well as for amino acids levels, there were Lumbricus rubellus (24.66%), Eisenia foetida (22.78%), Nereis sp. (18.37%). From the 15 amino acids detecte...

Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents

Canadian Journal of Infectious Diseases and Medical Microbiology, 2022

Aims. Current research aimed to explore the therapeutic values of different earthworms as antibacterial, anticoagulant, and antioxidant agents. Methods. Ten different earthworms, i.e., Amynthas corticis, Amynthas gracilis, Pheretima posthuma, Eisenia fetida, Aporrectodea rosea, Allolobophora chlorotica, Aporrectodea trapezoides, Polypheretima elongata, Aporrectodea caliginosa, and Pheretima hawayana, were collected and screened for biological activities. Antibacterial effect analysis of earthworm species was done against fourteen bacterial pathogens, i.e., Escherichia coli, Serratia marcescens, Streptococcus pyogenes, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa (1), Salmonella typhimurium, Shigella flexneri, Enterobacter amnigenus, Serratia odorifera, Pseudomonas aeruginosa (2), Staphylococcus warneri, and Lactobacillus curvatus, via agar well diffusion, crystal violet, MTT, agar disc diffusion, and direct bioautography assays. An...

EARTHWORM A POTENTIAL SOURCE FOR STABLE AND POTENT ANTIMICROBIAL

In the current work, we have isolated and purified an antimicrobial peptide from celomic fluid of Indian earthworm Pheretima posthumous. The purified peptide was characterized as serine protease and possesses broad range of antimicrobial activity. Another important finding was stability of antimicrobial peptide towards higher temperature, different pH and various inhibitors. The celomic fluid of earthworm was subjected to series of purification step such as ammonium sulphate precipitation, dialysis followed by size exclusion chromatography and ion exchange chromatography. The average molecular weight of peptide was found 20KD by SDS PAGE analysis. The peptide was evaluated for antibacterial and antifungal activity in various temperatures, pH and in the presence of different inhibitors. We have evaluated antibacterial activity of purified peptides among physchrophiles, mesophiles and thermophiles.

Therapeutic Uses of Earthworm – A Review

International Journal of Advanced Ayurveda, Yoga, Unani, Siddha and Homeopathy, 2020

Based on review of literature on Unani Medicine and contemporary scientific investigations, the study demonstrates enormous potential of common Indian earthworm to combat many human ailments. The information on clinical, pharmacological and chemical studies carried out on earthworms between 1930 and 2009 in India and abroad has been reviewed in an effort to substantiate their medical efficacy. In this paper, the methods of collection and processing of earthworms, their pharmaceutical composition, preliminary pharmacological and clinical effects on (i) nervous system; (ii) blood circulatory system; (iii) cardiovascular system; (iv) respiratory system; (v) uterus smooth muscle function; and (vi) anticancer properties are reviewed. Therapeutic efficacy of earthworms has been shown for tracheitis and bronchial asthma, epilepsy, high blood pressure, schizophrenia, leg ulcers, mumps, eczema, urticaria and anaphylaxis diseases, burns and scald fractures, erysipelas, sequel of encephalitisB, chronic lumbago, skin crevices, blood deficiency apoplexy, acute injury of soft tissues, vertigo, hematemesis and hematuria, digestive ulcer, vesicle calculus and cancer. Taking lead from the present reports detailed scientific investigations are needed to discover new therapeutic agents of zoological origin which might be very specific to treat certain diseases and conditions, thus far, incurable in modern medicine.

Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications

2013

Earthworms are invertebrates that have been widely applied to environmental and land study, however, a new chapter opens opportunities of research in cosmetic and medicine. The objectives of the experiment were to evaluate the nutritional and antioxidant properties from the earthworms Eudrilus eugeniae, Eisenia fetida and Lumbricus rubellus and to examine the bioactivity properties of the earthworms extract in tyrosinase, matrix metalloproteinase-1, and elastase inhibitory activities for cosmeceutical applications. The analyzed components include moisture, available carbohydrates, total lipids, dietary fiber, crude protein, amino acids, ash, and minerals. The amino acids profile of the earthworm extracts was evaluated using High Performance Liquid Chromatography (HPLC). Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-Page) was used to determine and separate the protein based on size. The results showed E. fetida had slightly higher amount of protein and ash than L. r...